Protein_Domain

Part:BBa_K4161602:Design

Designed by: Zhujun Yao 姚竹君   Group: iGEM22_DKU   (2022-09-27)


20ipaD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 258
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 258
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 258
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 258
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 258
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the sequence should be optimized in L.lactis. It should exclude specific restriction enzyme binding sites.


Source

The nanobody wasa designed in this paper:https://doi.org/10.1074/jbc.M117.802231

References